Epitope tags are frequently used with recombinant protein from E. coli, yeast or mammalian cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. Abbkine not only provides such as His tag, GST tag, S tag, HA tag, DDDDK tag, Myc tag, MBP Tag and mCherry tag monoclonal and polyclonal antibodies, but also offers Biotin, HRP, AbFluor™ dyes, agarose and magnetic beads conjugated antibodies.
Below I will overview in different tags for you.
| Product Name | Cat. No | Applications |
| Anti-DDDDK Tag Mouse Mab (1B10) | A02010 | WB, IF, IP |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 350 Conjugated | A02010A350 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 405 Conjugated | A02010A405 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 488 Conjugated | A02010A488 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 555 Conjugated | A02010A555 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 594 Conjugated | A02010A594 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 647 Conjugated | A02010A647 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), AbFluor™ 680 Conjugated | A02010A680 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), Cy3 Conjugated | A02010CY3 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), Cy5 Conjugated | A02010CY5 | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), FITC Conjugated | A02010FIT | IF |
| Anti-DDDDK Tag Mouse Mab (1B10), Agarose | A02010AGB | IP |
| Anti-DDDDK Tag Mouse Mab (1B10), Magnetic Beads | A02010MGB | IP |
| Anti-DDDDK Tag Rabbit Pab | A02011 | WB |
| Anti-DDDDK Tag Rabbit Pab, HRP Conjugated | A02011HRP | WB |
| HRP Conjugated Anti-DDDDK Tag Mouse Mab (1B10) | A02015 | WB |
2. Anti-HA Tag antibodies
| Product Name | Cat. No | Applications |
| Anti-HA Tag Mouse Mab (4F6) | A02040 | WB, IF, IP |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 350 Conjugated | A02040A350 | IF |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 405 Conjugated | A02040A405 | IF |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 488 Conjugated | A02040A488 | IF |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 555 Conjugated | A02040A555 | IF |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 594 Conjugated | A02040A594 | IF |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 647 Conjugated | A02040A647 | IF |
| Anti-HA Tag Mouse Mab (4F6), AbFluor™ 680 Conjugated | A02040A680 | IF |
| Anti-HA Tag Mouse Mab (4F6), Cy3 Conjugated | A02040CY3 | IF |
| Anti-HA Tag Mouse Mab (4F6), Cy5 Conjugated | A02040CY5 | IF |
| Anti-HA Tag Mouse Mab (4F6), FITC Conjugated | A02040FIT | IF |
| Anti-HA Tag Mouse Mab (4F6), Agarose | A02040AGB | IP |
| Anti-HA Tag Mouse Mab (4F6), Magnetic Beads | A02040MGB | IP |
| Anti-HA Tag Rabbit Pab | A02041 | WB |
| Anti-HA Tag Rabbit Pab, HRP Conjugated | A02041HRP | WB |
| HRP Conjugated Anti-HA Tag Mouse Mab (4F6) | A02045 | WB |
| Product Name | Cat. No | Applications |
| Anti-His Tag Mouse Mab (5C3) | A02050 | WB, IF, IP |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 350 Conjugated | A02050A350 | IF |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 405 Conjugated | A02050A405 | IF |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 488 Conjugated | A02050A488 | IF |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 555 Conjugated | A02050A555 | IF |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 594 Conjugated | A02050A594 | IF |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 647 Conjugated | A02050A647 | IF |
| Anti-His Tag Mouse Mab (5C3), AbFluor™ 680 Conjugated | A02050A680 | IF |
| Anti-His Tag Mouse Mab (5C3), Cy3 Conjugated | A02050CY3 | IF |
| Anti-His Tag Mouse Mab (5C3), Cy5 Conjugated | A02050CY5 | IF |
| Anti-His Tag Mouse Mab (5C3), FITC Conjugated | A02050FIT | IF |
| Anti-His Tag Mouse Mab (5C3), Agarose | A02050AGB | IP |
| Anti-His Tag Mouse Mab (5C3), Magnetic Beads | A02050MGB | IP |
| Anti-His Tag Rabbit Pab | A02051 | WB |
| Anti-His Tag Rabbit Pab, HRP Conjugated | A02051HRP | WB |
| HRP Conjugated Anti-His Tag Mouse Mab (5C3) | A02055 | WB |
4. Anti-V5 Tag antibodies
| Product Name | Cat. No | Applications |
| Anti-V5 Tag Mouse Mab (11D5) | A02170 | WB, IF, IP |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 350 Conjugated | A02170A350 | IF |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 405 Conjugated | A02170A405 | IF |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 488 Conjugated | A02170A488 | IF |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 555 Conjugated | A02170A555 | IF |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 594 Conjugated | A02170A594 | IF |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 647 Conjugated | A02170A647 | IF |
| Anti-V5 Tag Mouse Mab (11D5), AbFluor™ 680 Conjugated | A02170A680 | IF |
| Anti-V5 Tag Mouse Mab (11D5), Cy3 Conjugated | A02170CY3 | IF |
| Anti-V5 Tag Mouse Mab (11D5), Cy5 Conjugated | A02170CY5 | IF |
| Anti-V5 Tag Mouse Mab (11D5), FITC Conjugated | A02170FIT | IF |
| Anti-V5 Tag Mouse Mab (11D5), HRP Conjugated | A02170HRP | WB |
| Anti-V5 Tag Mouse Mab (11D5), Agarose | A02170AGB | IP |
| Anti-V5 Tag Mouse Mab (11D5), Magnetic Beads | A02170MGB | IP |
5. Anti-Myc Tag antibodies
| Product Name | Cat. No | Applications |
| Anti-Myc Tag Mouse Mab (2D5) | A02060 | WB, IF, IP |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 350 Conjugated | A02060A350 | IF |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 405 Conjugated | A02060A405 | IF |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 488 Conjugated | A02060A488 | IF |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 555 Conjugated | A02060A555 | IF |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 594 Conjugated | A02060A594 | IF |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 647 Conjugated | A02060A647 | IF |
| Anti-Myc Tag Mouse Mab (2D5), AbFluor™ 680 Conjugated | A02060A680 | IF |
| Anti-Myc Tag Mouse Mab (2D5), Cy3 Conjugated | A02060CY3 | IF |
| Anti-Myc Tag Mouse Mab (2D5), Cy5 Conjugated | A02060CY5 | IF |
| Anti-Myc Tag Mouse Mab (2D5), FITC Conjugated | A02060FIT | IF |
| Anti-Myc Tag Mouse Mab (2D5), Agarose | A02060AGB | IP |
| Anti-Myc Tag Mouse Mab (2D5), Magnetic Beads | A02060MGB | IP |
| Anti-Myc Tag Rabbit Pab | A02061 | WB |
| Anti-Myc Tag Rabbit Pab, HRP Conjugated | A02061HRP | WB |
| HRP Conjugated Anti-Myc Tag Mouse Mab (2D5) | A02065 | WB |
| Product Name | Cat. No | Applications |
| Anti-VSV-G-Tag Mouse Mab (14D2) | A02180 | WB, IF, IP |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 350 Conjugated | A02180A350 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 405 Conjugated | A02180A405 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 488 Conjugated | A02180A488 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 555 Conjugated | A02180A555 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 594 Conjugated | A02180A594 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 647 Conjugated | A02180A647 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), AbFluor™ 680 Conjugated | A02180A680 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), Cy3 Conjugated | A02180CY3 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), Cy5 Conjugated | A02180CY5 | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), FITC Conjugated | A02180FIT | IF |
| Anti-VSV-G-Tag Mouse Mab (14D2), HRP Conjugated | A02180HRP | WB |
| Anti-VSV-G-Tag Mouse Mab (14D2), Agarose | A02180AGB | IP |
| Anti-VSV-G-Tag Mouse Mab (14D2), Magnetic Beads | A02180MGB | IP |
7. Other Tag antibodies
| Product Name | Cat. No | Applications |
| Anti-GFP Tag Mouse Mab (3D3) | A02020 | WB, IP |
| Anti-GFP Tag Mouse Mab (3D3), HRP Conjugated | A02020HRP | WB |
| Anti-GFP Tag Rabbit Pab | A02021 | WB |
| Anti-GFP Tag Rabbit Pab, HRP Conjugated | A02021HRP | WB |
| Anti-GST Tag Mouse Mab (2A8) | A02030 | WB |
| Anti-GST Tag Mouse Mab (2A8), HRP Conjugated | A02030HRP | WB |
| Anti-MBP Tag Mouse Mab (9Y5) | A02070 | WB |
| Anti-MBP Tag Mouse Mab (9Y5), HRP Conjugated | A02070HRP | WB |
| Anti-mCherry Tag Mouse Mab (9D3) | A02080 | WB, IF |
| Anti-mCherry Tag Mouse Mab (9D3), HRP Conjugated | A02080HRP | WB |
| Anti-E2 Tag Mouse Mab (12T4) | A02090 | WB |
| Anti-E2 Tag Mouse Mab (12T4), HRP Conjugated | A02090HRP | WB |
| Anti-HSV Tag Mouse Mab (16T2) | A02100 | WB |
| Anti-HSV Tag Mouse Mab (16T2), HRP Conjugated | A02100HRP | WB |
| Anti-KT3 Tag Mouse Mab (14D8) | A02110 | WB |
| Anti-KT3 Tag Mouse Mab (14D8), HRP Conjugated | A02110HRP | WB |
| Anti-RFP Tag Mouse Mab (9D1) | A02120 | WB |
| Anti-RFP Tag Mouse Mab (9D1), HRP Conjugated | A02120HRP | WB |
| Anti-S Tag Mouse Mab (9T10) | A02130 | WB |
| Anti-S Tag Mouse Mab (9T10), HRP Conjugated | A02130HRP | WB |
| Anti-T7 Tag Mouse Mab (6D4) | A02150 | WB |
| Anti-T7 Tag Mouse Mab (6D4), HRP Conjugated | A02150HRP | WB |
| Anti-Trx Tag Mouse Mab (14D4) | A02160 | WB |
| Anti-Trx Tag Mouse Mab (14D4), HRP Conjugated | A02160HRP | WB |
| Anti-CBP Tag Mab (12H5) | A02190 | WB |
| Anti-CBP Tag Mab (12H5), HRP Conjugated | A02190HRP | WB |
| Anti-TAP Tag Mab (4H2) | A02200 | WB |
| Anti-TAP Tag Mab (4H2), HRP Conjugated | A02200HRP | WB |
| Anti-Avi-Tag Mab (5G11) | A02210 | WB |
| Anti-Avi-Tag Mab (5G11), HRP Conjugated | A02210HRP | WB |
| Anti-SRT-Tag Mab (11G3) | A02220 | WB |
| Anti-SRT-Tag Mab (11G3), HRP Conjugated | A02220HRP | WB |
| Anti-mStrawberry Mab (4C9) | A02240 | WB |
| Anti-mStrawberry Mab (4C9), HRP Conjugated | A02240HRP | WB |
| Anti-EYFP-Tag Mab (10T3) | A02250 | WB |
| Anti-EYFP-Tag Mab (10T3), HRP Conjugated | A02250HRP | WB |
| Anti-mOrange Mab (9A10) | A02260 | WB |
| Anti-mOrange Mab (9A10), HRP Conjugated | A02260HRP | WB |
| Anti-AmCyan Mab (8T2) | A02270 | WB |
| Anti-AmCyan Mab (8T2), HRP Conjugated | A02270HRP | WB |
| Anti-ECFP-Tag Mab (6B11) | A02280 | WB |
| Anti-ECFP-Tag Mab (6B11), HRP Conjugated | A02280HRP | WB |
| Anti-EBFP Mab (8B5) | A02290 | WB |
| Anti-EBFP Mab (8B5), HRP Conjugated | A02290HRP | WB |
| Anti-Nano-Tag9 Mab (11T3) | A02300 | WB |
| Anti-Nano-Tag9 Mab (11T3), HRP Conjugated | A02300HRP | WB |
How to choose an epitope?
* The best known tag is poly-histidine, which is based upon the affinity of nickel for four or more consecutive histidine residues. It is popular because of its ability to purify under denaturing conditions, ease of reuse, and reasonable price.
* Tags that use antibody-based purification formats (such as DDDDK, HA, HSV, c-Myc) offer higher levels of purity, but the antibody resins are expensive to produce and cannot be reused easily. This is of particular use in high-throughput screening, or where proteins are expressed at low levels.
* Other tags with cost-effective purification resins are MBP and GST. These tags are approximately 40 times the size of metal affinity tags, so they may interfere with the structure or function of the fusion partner. However, tags such as MBP and GST can improve solubility, which is especially important when expressing proteins at high levels in prokaryotes as they have more primitive posttranslational folding and processing machinery than eukaryotes.
* Epitope tags can also be used for immobilization or attachment, to investigate protein interactions. If a purification resin is loaded with protein, then it can be used as ‘bait’ to capture binding partners (immunoprecipitation). In this case, the specifcity of the resin becomes crucial to avoid interference from contaminants.
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