In this free webinar, the featured speaker will give an overview of bioanalysis of protein therapeutics using a surrogate peptide approach. He will discuss case study results for the preclinical bioanalysis of a humanized IgG protein therapeutic by hybrid IA-LC-MS/MS. Attendees will learn about practical tips for optimizing immunoaffinity enrichment and trypsin digestion workflows.
TORONTO (PRWEB) June 09, 2020
Bioanalysis of protein therapeutics by LC-MS/MS continues to gain popularity as a complementary approach to ligand-binding assays (LBA). LC-MS/MS offers multiple advantages over LBA including higher specificity, multiplexing capability and reduced development time. Immunoaffinity enrichment can be coupled to LC-MS/MS to produce a hybrid method that increases sensitivity and selectivity.
This webinar will highlight the systematic optimization of a flexible, hybrid IA-LC-MS/MS workflow that uses streptavidin-coupled magnetic beads, ’rapid’ trypsin digestion and surrogate peptide quantification.
Key areas of focus will include:
- The selection of streptavidin-coupled magnetic beads as the platform for the immunoaffinity enrichment system due to their amenability to use in combination with analyte-specific biotinylated capture reagents
- A workflow case study for a humanized antibody therapeutic in preclinical serum using generic conserved surrogate peptides for quantification that, when optimized, ultimately requires only ~3 hours with minimal direct handling
- Additional applications for the optimized workflow, such as highly selective and sensitive analysis of humanized antibody in preclinical samples and protein therapeutics using analyte-specific biotinylated capture reagents
For more information or to register for this event, visit Protein Therapeutic Bioanalysis by Hybrid IA-LC-MS/MS: Optimization of a Preclinical Antibody Workflow.
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For the original version on PRWeb visit: https://www.prweb.com/releases/protein_therapeutic_bioanalysis_by_hybrid_ia_lc_ms_ms_optimization_of_a_preclinical_antibody_workflow_upcoming_webinar_hosted_by_xtalks/prweb17175660.htm